31th ISTA Congress Poster - Establishment of Tetrazolium Testing Procedure of Papaya (Carica papaya L.) Seed v1.2.pdf - 農業知識入口網

Establishment of Tetrazolium Test Procedure of Papaya
(Carica papaya L.) Seed
I-Cheng Chen1, Yu-Mei Huang1, Wen-Ju Yang2 and Tso-Chi Yang1
1 Taiwan Seed and Seedling Improvement and Propagation Station (TSIPS), C. O. A.
2 Department of Horticulture and Landscape Architecture, National Taiwan University
Introduction C. Staining duration Extending the staining duration from
3 to 6 hours shows no significant difference on permitted
Papaya (Carica papaya L.) is an important fruit crop and
viable papaya seeds (Table 3).
the main kind of fruit seed exportation from Taiwan. But the
Table 3. Comparison of viable seeds percentage of papaya
tetrazolium test of papaya seed is not included in the
seed with different staining durations.
International Seed Testing Rules. This study tested the
Staining duration (hours) Viable seeds (%)
tetrazolium (TTC) testing conditions of papaya seed to quickly
3 86 a
estimate seed viability.
4 79 a
Material and Methods 5 79 a
6 82 a
Significance Level: 0.05
(2) Evaluation of Viable and Non-viable Tissue
Six staining types were separated by different staining
patterns of seven papaya seed lots in this study (Figure.1)
and calculated the RMS with germination rates. The result
shows the combination of staining types 1, 2 and 3 calculated
the lowest RMS of 3.83 (data not shown) indicating the
combination meet the seed germination rate (Table 4).
Result and Discussion
(1) Establishment of Tetrazolium Test Procedure
A. Premoistening duration The result shows the effect of
Figure 1. The TTC staining types of papaya seed.
extending the premoistening duration from 18 to 48 hours
on viable seed evaluation is not significant (Table 1).
Table 4. Comparison of TTC permitted viability and germination rate of
papaya seeds.
Table 1. Comparison of viable seeds percentage of papaya
seed with different premoistening durations.
Staining Type Lot 1 Lot 2 Lot 3 Lot 4 Lot 5 Lot 6 Lot 7
Premoistening duration (hours) Viable seeds (%)
Type 1 17.0 68.5 58.5 20.5 41.0 69.0 77.0
18 86 a
Type 2 11.5 11.5 21.5 18.5 29.0 12.5 10.5
24 78 a
Type 3 3.5 4.0 1.0 3.0 3.5 7.0 1.5
48 72 a
Type 4 4.0 0 1.0 1.0 1.0 3.5 0.5
Significance Level: 0.05
Type 5 7.5 8.0 1.5 11.0 0.5 0 2.5
B. Staining solution concentration There was no
Type 6 56.5 8.0 16.5 46.0 25.0 8.0 8.0
statistics difference between two different staining solution
concentration (0.5% and 1 %) on viable seeds permitting Permitted Viability (%) 32.0az 84.0a 81.0a 42.0a 73.5a 88.5a 89.0b
of two papaya seed lots with different seed vigor (Table 2).
Germination Rate (%) 33.3a 84.8a 75.0a 40.5a 76.3a 85.0a 95.5a
z Means within the same letters in a column are not significantly different by
Table 2. Comparison of viable seeds percentage of papaya
Fisher’s LSD test at 5% level.
seed with different staining solution concentrations.
TTC Concentration (%) Viable seeds (%)
corresponding author: I-Cheng Chen
Lot 1 Lot 2
e-mail: ICChen@tss.gov.tw
0.5 % 78.3 a 35.0 a
tel: +886-4-25825488
1.0 % 75.0 a 30.0 a
fax: +886-4-25825439
Significance Level: 0.05